Device
Part:BBa_K1329000:Design
Designed by: Daniela Geist Group: iGEM14_Marburg (2014-10-01)
StrepDARPidin
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 598
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We designed the construct with an N-terminal His-Tag for purification and Antibody targeting. GS-linkers were used to give the construct flexibility to provide steric tensions.
Source
The sequence for streptavidin was found in the Streptomyces avidinii genome (http://blast.st-va.ncbi.nlm.nih.gov/Blast.cgi#alnHdr_134951). The sequence was synthesized and optimized for E. Coli codon usage. The aminoacid sequence of DARPin Ec1 was found in the supplementary materials of the publication "DARPins Recognizing the Tuor-Associated Antigen EpCAM Selected by Phage and Ribosome Display and Engineered for Multivalency". The sequence was synthesized and optimized for E. Coli codon usage.