Device

Part:BBa_K1329000:Design

Designed by: Daniela Geist   Group: iGEM14_Marburg   (2014-10-01)


StrepDARPidin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 598
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We designed the construct with an N-terminal His-Tag for purification and Antibody targeting. GS-linkers were used to give the construct flexibility to provide steric tensions.


Source

The sequence for streptavidin was found in the Streptomyces avidinii genome (http://blast.st-va.ncbi.nlm.nih.gov/Blast.cgi#alnHdr_134951). The sequence was synthesized and optimized for E. Coli codon usage. The aminoacid sequence of DARPin Ec1 was found in the supplementary materials of the publication "DARPins Recognizing the Tuor-Associated Antigen EpCAM Selected by Phage and Ribosome Display and Engineered for Multivalency". The sequence was synthesized and optimized for E. Coli codon usage.

References